Partial purification of peroxiredoxin-2 from porcine skeletal muscle
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چکیده
منابع مشابه
Purification of dystrophin from skeletal muscle.
Dystrophin was purified from rabbit skeletal muscle by alkaline dissociation of dystrophin-glycoprotein complex which was first prepared by derivatized lectin chromatography. Dystrophin-glycoprotein complex was isolated from digitonin-solubilized rabbit skeletal muscle membranes by a novel two-step method involving succinylated wheat germ agglutinin (sWGA) chromatography and DEAE-cellulose ion ...
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Skeletal muscle contains multiple progenitor populations of distinct embryonic origins and developmental potential. Myogenic progenitors, usually residing in a "satellite cell position" between the myofiber plasma membrane and the laminin-rich basement membrane that ensheaths it, are self-renewing cells that are solely committed to the myogenic lineage. We have recently described a second class...
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Integral membrane-associated arginine-specific mono-ADP-ribosyltransferase was purified from rabbit skeletal muscle microsomes. The ADP-ribosyltransferase was solubilized from the 100,000 x g pellet with 0.3% sodium deoxycholate and purified to greater than or equal to 95% homogeneity by successive DE52, concanavalin A-agarose, 3-aminobenzamide-agarose, and size-exclusion high-performance liqui...
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A procedure has been devised for isolation of triads (t-tubule/sarcoplasmic reticulum (SR) junctional complexes) from rabbit skeletal muscle. The procedure consists of preparation of a heavy microsomal fraction followed by two sequential 90-min sucrose gradient centrifugations to enrich the triads. A pyrophosphate/phosphate/magnesium buffer system was introduced to decrease aggregation in order...
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Adenylosuccinate synthetase has been purified more than 250-fold from extracts of rabbit muscle acetone powder by heating to 60”, ammonium sulfate fractionation, phosphocellulose and hydroxylapatite chromatography, and Sephadex G-150 gel filtration. Crystals have been obtained by dialysis against 60 to 67% ammonium sulfate. Enzyme activity is stable for about 2 weeks in phosphate buffer contain...
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ژورنال
عنوان ژورنال: Meat and Muscle Biology
سال: 2021
ISSN: 2575-985X
DOI: 10.22175/mmb.12408